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IncuCyte® Chemotaxis System for Directional Migration Assays

Thursday, February 22, 2018   (0 Comments)
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To measure directional migration, the Transwell® Boyden chamber has been widely regarded as the mainstay in vitro method. However, it is known for being technically tricky, hard to troubleshoot, and frequently yielding variable data.

The new IncuCyte® Chemotaxis System from Essen Bioscience offers a robust walkaway, fully kinetic and image-based solution in 96-well plates. The ClearView 96-well plate consumable consists of an ordered array of 8 μm pores on a viewing surface in each well.

The IncuCyte® live-cell analysis system is used to visualize the directional migration of cells across the surface and toward chemoattractant positioned in the reservoir of the plate and IncuCyte® Chemotaxis Cell Migration image analysis software is used to quantify this migration. This article discusses the validation of this integrated solution for adherent as well as non-adherent cell types.

Major advantages include:

  • Full visualization of the cell biology
  • Low cell usage
  • Easier workflows
  • Relevant surface biology
  • Highly reproducible 96-well data

Read more.

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